ABSTRACT
OBJECTIVE: This study aimed to assess miRNA-195 expression in the tumor tissues from a cohort of Brazilian female breast cancer patients undergoing neoadjuvant chemotherapy (NAC) and evaluate its correlation with various clinicopathological markers. METHODS: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate the miRNA-195 expression in tumor tissues from a cohort of female breast cancer patients undergoing NAC. This expression was then correlated with the occurrence of several distinct breast cancer molecular subtypes and other clinicopathological variables. RESULTS: A total of 55 patients were included in this study, 28 (50.9%) of whom were treated using NAC. Tumor miRNA-195 expression was suppressed in breast cancer patients, regardless of their exposure to systemic treatments, histological grade, size, nodal status, and tumor-node-metastasis (TNM) staging. This was more pronounced in luminal and triple-negative patients, and patient's response to NAC was correlated with an increase in miRNA-195 expression. CONCLUSION: miRNA-195 is downregulated in the tumor tissues of Brazilian breast cancer patients regardless of NAC exposure; this reinforces its role as a tumor suppressor and a potential biomarker for chemotherapy response.
Subject(s)
Humans , Female , Breast Neoplasms/genetics , Breast Neoplasms/pathology , Breast Neoplasms/drug therapy , MicroRNAs/genetics , Prognosis , Brazil , Antineoplastic Combined Chemotherapy Protocols , Biomarkers, Tumor/genetics , Neoadjuvant Therapy , Neoplasm StagingABSTRACT
Objective To investigate the relationship between the expression of plasma miRNA-195 in pa-tients with severe coronary artery stenosis and its coronary collateral circulation. Methods One hundred and nine-ty patients with selective coronary angiography in our hospital from March 2000 to May 2017 were enrolled,who were divided into poor collateral circulation group(n = 90)and good collateral circulation group(100 cases)ac-cording to the Rentrop classification.Plasma miRNA-195 and VEGF levels were compared between the two groups. The relationship between plasma miRNA-195 and VEGF was analyzed,and also the relationship between plasma miRNA-195 and Gensini points.Results(1)Plasma miRNA-195 and VEGF levels in good collateral circulation group were significantly higher than those in poor collateral circulation group(P<0.05).(2)There was a positive correlation between plasma miRNA-195 and VEGF in patients with coronary heart disease(r=0.628,P=0.022). (3)There was a positive correlation between plasma miRNA-195 and Gensini score in patients with coronary heart disease(r = 0.765,P = 0.013). Conclusion MiRNA-195 may be involved in the production of neovasculariza-tion by regulating VEGF in the formation of collateral circulation in patients with severe coronary artery stenosis, which can be used as a biomarker for predicting the formation of collateral circulation for clinical reference.